Product Name: aSMART™ TMB Peroxidase (HRP) ELISA Substrate
Catalogue No.: 561001
Description: This Peroxidase Microwell Substrate contains 3,3’,5,5’-Tetra Methyl Benzidine (TMB) in an optimized buffer system. The substrate formulation is manufactured as a colorless liquid in appearance. When TMB ARC-TREME is reacted with peroxidase, a reaction ensues producing a blue colored liquid product (oxidized state). ARC’s TMB-BlueSTOP may be used to stop the reaction at this “blue stage”, to read at 650nM. The reaction also can be terminated more typically using an appropriate reagent (an acid) or ARC’s TMB-YellowStop. By stopping the reaction in this manner, a second oxidized state is produced resulting in a soluble yellow reaction product. Depending on the stop reagent utilized, the colored product is stable for at least two hours.
Format: Provided as a ready to use one component solution.
Chemical Hazard: None. This product does not contain any caustic solvents
Uses: The product should be equilibrated to room temperature (25˚C) before use. For immunoassay microwell applications, 0.1mL of substrate solution should be added to each well. A liquid soluble blue reaction develops which can be read at approximately at 370nm or between 620nm and 650nm. Optimally, absorbance values should be monitored before they exceed 2.0 OD units. In endpoint assays and for best results, the ARC’s TMB-Yellow Stop reagent may be used. The substrate reaction also can be stopped using equal volumes of 1N HCL or 0.6N Sulfuric Acid. Addition of Yellow Stop or acid turns the blue color to yellow at end point. Absorbance values increase approximately three fold at end point. Hence, sample OD values should be monitored and the substrate reaction terminated when values reach approximately 0.7 OD units. For ARC’s TMB-Yellow Stop or an acid Yellow Stop a liquid soluble yellow reaction develops which is read in the 450nm. Dilution of the substrate is not recommended and will alter its performance characteristics. To reduce reaction kinetics, it is recommended that analytes, antibodies or conjugates used in the assay, be diluted. This product is light sensitive and should be protected from direct sunlight or UV sources. For laboratory research use only.
Storage: Recommended to store at 2° to 8°C in refrigerator. DO NOT FREEZE.
Shelf Life: Stable for a minimum of two years if stored at recommended conditions.
Shipping: Products may be shipped at room temperature or on ice pack.