aSMART™ TMB Peroxidase (HRP) ELISA Substrate

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Product Name: aSMART™ TMB Peroxidase ELISA Substrate

Catalogue No.: 561001

Description: aSMART™ TMB Peroxidase ELISA Substrate is a stabilized one component substrate that can detect horseradish peroxidase enzyme activity at high sensitivity. Our product also stable with long shelf life, 4 years.

Format: Provided as a ready to use one component reagent solutions in amber bottle. It is colorless and appear clear.

Chemical Hazard: This product does not contain any caustic strong acids and other wise safe to
use. Do not contain DMSO or DMF.

Uses: Recommended for use with ELISA in any format, or Similar applications.

Method of Use: Add 100 uL of this substrate reagent directly to each washed and drained wells of micro plate using a multichannel pipettor. Incubate for 5 to 10 minutes, not more than 10 minutes. Read the plate at 620nM using a microplate reader. Or stop the reaction by adding equal volume of a stop reagent (Item 561002) and read the plate immediately at 450nM using a microplate reader. Reagent removed from the bottle should not be poured back to avoid contamination.

Storage: Recommended to store at 2°C to 8°C in dark in a refrigerator.

Shelf Life: This product has a shelf life of 48 months (4 years) when stored in the dark at 2◦C to 8◦C as recommended.

Shipping: Products may be shipped on ice pack.