Product: Human MDA-MB-231 Cell Line
Catalog No.: 116088
Description: The cell line is suitable for transfection studies. It expresses receptors for EGF and TGF alpha.
Type: Human metastatic adenocarcinoma cell line
Source: Human breast cancer
Amount: 1e6 cells/vial in a frozen vial or about 5x 1e6 cells in a T25 flask.
Format: Provided as a frozen vial in our freeze media or as actively growing culture in a T25 flask.
Morphology: Epithelial
Growth Mode: Adherent monolayer
Growth Media: Recommended media is Leibovitz’s L-15 media with 10% Fetal Calf Serum. DMEM with 10% Fetal Calf Serum may also be used. Culture incubates with 5% CO2; at 37°C.
Subculture: Split culture using 0.25% Trypsin when about 80% confluent and re-seed at about 4000 cells per sq.cm.
Uses: For virus titration, generation of recombinant cell lines and protein production. For laboratory research use only.
Storage: Store frozen vials in liquid nitrogen at -196˚C. Do not store at -80˚C freezer.
Shipping: Products may be shipped on dry ice if frozen. On special request, actively growing cells in T25 flasks may be shipped at room temperature (additional cost may apply).
