4F10 Cell Line (Hybridoma)

$655.00
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SKU:
116053
Size:
Vial
4F10 Cell Line (Hybridoma)

Product: 4F10 Cell Line (Hybridoma)

Catalog No.: 116053

Description: 4F10 cell line is a spontaneous clonal switch variant of the murine B cell line. These are membrane IgA and spontaneously secrete small amounts of IgA which is mostly monomeric.

Amount: ~5 x 10e6 cells per vial per ml in our freezing media (ARC Catalog# 113026). 

Organism: Mouse

Tissue Type: Mouse Hybridoma 

Phenotype: Non-adherent cells.

Format: Provided as a frozen vial of cells containing about 5,000,000 cells/vial/mL in our freezing media (ARC Catalog# 113026). 

Growth Media: DMEM with 10% FCS or similar. We highly recommended to use ‘HyMax™ Hybridoma Fusion and Cloning Supplement’ (our Catalog# 113004) to promote growth and viability of lymphocytes.

Storage: On receipt it recommended to transfer frozen vial immediately to liquid Nitrogen for storage. Or thaw vial as per standard cell culture procedure and initiated into a cell culture in T25 flask or similar. Do not store frozen cell lines at -80◦C freezer.

Shipping: This product may be shipped on dry ice as a frozen vial. On special request, actively growing cells in T25 flasks may be shipped at room temperature (additional cost may apply).