Description
Product: Mouse L929 Cell Line
Catalog No.: 116017
Description: This is a subclone of ‘L’ cells originally established by W R Earle in 1940. It was derived from normal subcutaneous tissue of a 100-day old male C3H/An mouse.
Type: Mouse connective tissue established cell line
Source: Mouse skin tissue
Amount: 5x 1e6 cells/vial in a frozen vial or about 5x 1e6 cells in a T25 flask.
Format: Provided as a frozen vial in our freeze media or as actively growing culture in a T25 flask.
Morphology: Fibroblasts
Growth Mode: Adherent monolayer
Growth Media: DMEM with 5%-10% Fetal Calf Serum. Incubate in 5% CO2; at 37°C.
Subculture: Split culture using 0.25% Trypsin when about 80% confluent and re-seed at about 4000 cells per cm2.
Uses: For virus titration, generation of recombinant cell lines and protein production. For laboratory research use only.
Storage: Store frozen vials in liquid nitrogen at -196˚C. Do not store at -80˚C freezer.
Shipping: Products may be shipped on dry ice if frozen. On special request, actively growing cells in T25 flasks may be shipped at room temperature (additional cost may apply).
