Anti Bromodeoxyuridine (BrdU) (Proliferation Marker) Mab [BRD494]

Product Name: Anti Bromodeoxyuridine (BrdU) (Proliferation Marker) Mab [BRD494]

Catalogue No.: 631242

Alternate Names: Bromodeoxyuridine, BUdr

Description: It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Immunogen: Bromodeoxyuridine (BrdU) conjugated to KLH

Clonality: Monoclonal

Host: Mouse

Specificity: All Species

Concentration: 0.2mg/mL

Purity: >90% pure by SDS PAGE

Format: Provide in PBS with 0.05% BSA and 0.05% sodium azide

Applications: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml);
Immunohistology (Frozen & Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
[For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC]
Optimal dilution for a specific application should be determined.

Stability: Stable for 24 months is stored as recommended.

Storage: Store at 2-8°C refrigerator for short term use. Aliquot and store at -20°C or –
80°C for long term. Minimize freezing and thawing.

Shipping: Products may be shipped on ice pack.