Product: BV2 Cell line
Catalog No.: 116073
Description: BV2 is a neuronal glial cell line generated from mouse brain tissue by recombinant retrovirus J2 transformation. This cell line is widely used in research especially used in brain inflammation studies. We have this cell line available in frozen format for cell culture or protein extract for western blot applications.
Amount: 5e6 cells per vial per ml in freezing media.
Certifications: Certified negative for HIV, HBV, HCV, Mycoplasma, Bacteria, Fungi, Yeast
Organism: Mouse, Mus musculus C57BL/6 female
Tissue: Brain, glial cells
Morphology: Microglial
Phenotype: Mixed Adherent and suspension when grown in standard media
Biosafety Level: 1
Format: Frozen vial of cells containing 5,000,000 cells/vial/mL in freezing media. Available as actively growing T25 flask culture, on request.
Growth Media: DMEM with 10% FCS or similar.
Culture Conditions: Follow standard cell culture procedures and precautions. Use trypsin-EDTA to prepare single cell suspension. Subculture or split cells 1:4 about every 3 days. Use incubator set at 5% CO2 and 37◦C for growth.
Storage: Upon receipt it recommended to transfer vial immediately to liquid Nitrogen for storage. Or thaw vial as per standard cell culture procedure and initiated into a cell culture in T25 flask or similar. Do not store at -80◦C freezer.
Shipping: Products may be shipped on dry ice.On special request actively growing cells in T25 flask may be shipped at room temperature (additional cost may apply).
