Product: BW1100 Cell line (T Cell Thymoma)
Catalog No.: 116031
Description: BW1100 cell line is widely used in research for generation of T cell hybridomas. It is deficient in TcR receptors.
Amount: 5 x 10e6 cells per vial per ml in our freezing media (ARC Catalog# 113026). If flask culture expect 2x 1e6 to 3x 10e6 cells at 50% confluency in a T25 flask.
Organism: Mouse
Tissue Type: Thymus derived T cells
Phenotype: Adherent lightly, if grown in standard culture media containing serum.
Format: Provided as a frozen vial of cells containing 5,000,000 cells/vial/mL in our freezing media (ARC Catalog# 113026). If a flask culture expect to be 50% confluent with DMEM media with 10% fetal calf serum and standard antibiotics.
Growth Media: DMEM with 10% FCS or similar. We highly recommended to use ‘HyMax Hybridoma Fusion and Cloning Supplement’ (our Catalog# 113004) to promote growth and viability of lymphocytes.
Storage: Upon receipt it recommended to transfer frozen vial immediately to liquid Nitrogen for storage. Or thaw vial as per standard cell culture procedure and initiated into a cell culture in T25 flask or similar. If flask culture transfer to CO2 incubator for continued culturing after removing excess media. Do not store frozen cell lines at -80◦C freezer.
Shipping: Products may be shipped on dry ice if frozen vial. On special request actively growing cells in T25 flask may be shipped at room temperature (additional cost may apply).
