Product: HEK293 Cell line (Human Embryonic Kidney Cells)
Catalog No.: 116007
Description: Human Embryonic Kidney (HEK) 293 is a cell line commonly used for BioPharma and Basic Medical research and therapeutic solutions. Generated in 1973, they are derived from human embryonic kidney cells and are popular, in part because of their reliable growth in culture and for their propensity for transfection.
Amount: 5 x 10e6 cells per vial per ml in freezing media. If flask culture expect 2e6 to 3e6 cells at 50% confluency.
Organism: Human
Tissue Type: Epithelial from embryonic kidney.
Phenotype: Adherent, if grown in standard culture media containing serum.
Format: Provided as a frozen vial of cells containing 5000,000 cells/vial/mL in freezing media. Freezing media contains 10% DMSO and 40% FCS and 50% DMEM. If a flask culture expect to be 50% confluent with DMEM media with 10% fetal calf serum.
Growth Media: DMEM with 10% FCS or similar.
Storage: Upon receipt it recommended to transfer frozen vial immediately to liquid Nitrogen for storage. Or thaw vial as per standard cell culture procedure and initiated into a cell culture in T25 flask or similar. If flask culture transfer to CO2 incubator for continued culturing after removing excess media. Do not store at -80°C freezer.
Shipping: Products may be shipped on dry ice if frozen vial. On special request actively growing cells in T25 flask may be shipped at room temperature (Additional cost may apply).
