Product: HeLa Cell line (Human Cervical Carcinoma)
Catalog No.: 116008
Description: HeLa cell line is a transformed Human Embryonic Kidney cell line. This cell line is widely used in research.
Amount: If frozen vial expect 5 x 10e6 cells per vial per ml in freezing media. If flask culture expect 3x 10e6 cells at 50% confluency.
Organism: Human
Tissue Type: Epithelial from Human cervix
Phenotype: Adherent, if grown in standard culture media containing serum
Format: Provided as a frozen vial of cells containing 5,000,000 cells/vial/mL in freezing media. Freezing media contains 10% DMSO and 40% FCS and 50% DMEM. Actively growing flask culture of cells may be requested (for additional cost). If a flask culture expect to be 50% confluent cells with DMEM media with 10% fetal calf serum.
Growth Media: DMEM with 10% FCS or similar.
Storage: Up on receipt it recommended to transfer frozen vial immediately to liquid Nitrogen for storage. Or thaw vial as per standard cell culture procedure and initiated into a cell culture in T25 flask or similar. If flask culture transfer to CO2 incubator for continued culturing after removing excess media. Or culture may be split depending on cell density. Do not store at -80◦C freezer.
Shipping: Products may be shipped on dry ice if frozen vial. On special request actively growing cells in T25 flask may be shipped at room temperature (additional cost may apply).
