Product: Hep2 Cell Line (Human larynx squamous cell carcinoma)
Catalog No.: 116009
Description: The HEp-2 cell line originated from tumors produced after injection of rats with epidermoid carcinoma tissue. They are currently one of the most common substrates for antinuclear antibody detection by immunofluorescence.
Amount: If frozen vial expect 5 x 10e6 cells per vial per ml in freezing media. If flask culture expects 3x 10e6 cells at 50% confluency.
Organism: Human.
Tissue Type: Larynx squamous cell carcinoma.
Phenotype: Adherent, if grown in standard culture media containing serum.
Format: Provided as a frozen vial of cells containing 5,000,000 cells/vial/mL in freezing media. Freezing media contains 10% DMSO and 40% FCS and 50% DMEM. Actively growing flask culture of cells may be requested (for additional cost). If a flask culture expects to be 50% confluent cells with DMEM media with 10% fetal calf serum.
Growth Media: DMEM with 10% FCS or similar.
Storage: Upon receipt it recommended to transfer frozen vial immediately to liquid Nitrogen for storage. Or thaw vial as per standard cell culture procedure and initiated into a cell culture in T25 flask or similar. If flask culture transfer to CO2 incubator for continued culturing after removing excess media. Or culture may be split depending on cell density. Do not store at -80◦C freezer.
Shipping: Products may be shipped on dry ice if frozen vial. On special request, actively growing cells in T25 flask may be shipped at room temperature (additional cost may apply).
