Product: NIH3T3 (NIH 3T3) Cell Line (Mouse embryonic fibroblast)
Catalog No.: 116022
Description: The embryonic mouse fibroblast cell line NIH3T3 is widely used in life science research, including the study of cell cycle control and primary cilia. Fibroblasts are the most important cell type in connective tissue, as they produce components of the extracellular matrix and determine tissue architecture.
Amount: ~5 x 10e6 cells per vial per ml in our freezing media (ARC Catalog# 113026).
Organism: Mouse
Tissue Type: Embryonic fibroblast cell
Phenotype: Non-adherent cells.
Format: Provided as a frozen vial of cells containing about 5,000,000 cells/vial/mL in our freezing media (ARC Catalog# 113026).
Growth Media: DMEM with 10% FCS or similar. We highly recommended to use ‘HyMax™ Hybridoma Fusion and Cloning Supplement’ (our Catalog# 113004) to promote growth and viability of lymphocytes.
Storage: On receipt it recommended to transfer frozen vial immediately to liquid Nitrogen for storage. Or thaw vial as per standard cell culture procedure and initiated into a cell culture in T25 flask or similar. Do not store frozen cell lines at -80◦C freezer.
Shipping: This product may be shipped on dry ice as a frozen vial. On special request, actively growing cells in T25 flasks may be shipped at room temperature (additional cost may apply).
