L929 cell line (mouse subcutaneous connective tissue)

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L929 cell line   (mouse subcutaneous connective tissue)

Product Name: Mouse L929 Cell Line

Catalogue No.: 116017

Description: This is a subclone of ‘L’ cells originally established by W R Earle in 1940. It was
derived from normal subcutaneous tissue of a 100 day old male C3H/An mouse.

Type: Mouse connective tissue established cell line

Source: Mouse skin tissue

Amount: 5e6 cells/vial

Format: Provided as a frozen vial in our freeze media

Morphology: Fibroblasts

Growth Mode: Adherent monolayer

Growth Media: DMEM with 10% Fetal Calf Serum. Incubate in 5% CO2; at 37°C.

Subculture: Split culture using 0.25% Trypsin when about 80% confluent and re-seed at
about 4000 cells per cm2.

Uses: For virus titration, generation of recombinant cell lines and protein production.
For laboratory research use only.

Storage: Store frozen vials in liquid nitrogen at -196˚C. Do not store at -80˚C freezer.

Shipping: Products may be shipped on dry ice.