Product: 4F10 Cell Line (Hybridoma)
Catalog No.: 116053
Description: 4F10 cell line is a spontaneous clonal switch variant of the murine B cell line. These are membrane IgA and spontaneously secrete small amounts of IgA which is mostly monomeric.
Amount: ~5 x 10e6 cells per vial per ml in our freezing media (ARC Catalog# 113026).
Organism: Mouse
Tissue Type: Mouse Hybridoma
Phenotype: Non-adherent cells.
Format: Provided as a frozen vial of cells containing about 5,000,000 cells/vial/mL in our freezing media (ARC Catalog# 113026).
Growth Media: DMEM with 10% FCS or similar. We highly recommended to use ‘HyMax™ Hybridoma Fusion and Cloning Supplement’ (our Catalog# 113004) to promote growth and viability of lymphocytes.
Storage: On receipt it recommended to transfer frozen vial immediately to liquid Nitrogen for storage. Or thaw vial as per standard cell culture procedure and initiated into a cell culture in T25 flask or similar. Do not store frozen cell lines at -80◦C freezer.
Shipping: This product may be shipped on dry ice as a frozen vial. On special request, actively growing cells in T25 flasks may be shipped at room temperature (additional cost may apply).
