HyMAX™ Hybridoma Fusion and Cloning Supplement, 20X
Catalog No.: 113004
Alternate Names: Hybridoma supplement, Hybridoma cloning factor, HCF, hybridoma cloning supplement, HCS, hybridoma cloning & fusion supplement, HCFS, hybridoma growth supplement, HGS, hybridoma fusion and cloning booster, hybridoma fusion and cloning supplement, HFCS
Description: HyMax™ Hybridoma Fusion and Cloning Supplement is a blended supplement media containing various cytokines, growth factors, hormones and vitamins specifically formulated for the survival and clonality of newly developing hyridoma. It is formulated to replace feeder cells during new hybridoma development. It can also be used for fast recovery of frozen cells from liquid nitrogen. It also found to stabilize antibody production by hybridoma, while untreated may lose IgG titer.
Feeder cells are routinely used during plating of cells after fusion. Typically, feeder cells provide many unknown growth factors to the newly developing clones. These includes many cytokines which are involved in isotype switching and maintenance of IgG secretion by B-cells. From our many years of experience with hybridoma development we have formulated this hybridoma media supplement that support better growth & higher frequency of IgG producing clones. It is tested for hybridoma colony forming efficiency and maintenance of IgG secretion.
Usage: Add 5mL of HyMax™ 20x to 95mL of your working media currently used for culturing cells to make 5%. Do not use HyMax™ at more than 10%. Continue with culturing cells with this media until cells have grown well for few generations. Once cells have grown well, culture may be weaned off of HyMax™ by gradually reducing the percentage in steps to 1%, 0.5% and 0%.
Format: Provided as a sterile filtered, tissue culture tested, 20x concentrate ready to use
solution. Never frozen before shipping to customer. Each 100mL bottle is enough to make 2 liter of working media, enough for doing a complete hybridoma development project.
Storage: It is recommended to store at 4°C - 8°C in refrigerator until use within 6 months. It
can be made into aliquots and frozen stored at -20°C - 80°C for long term to use within a 12-month period. Avoid repeated freezing and thawing, which may cause activity loss and or show precipitation.
Stability: 6 months when stored at 4°C - 8°C in refrigerator or 12 months when stored frozen.
Once thawed use within 30 days or aliquot and store as needed. Do not freeze & thaw repeatedly.
Shipping: It is prepared fresh (within 90 days) and shipped in liquid form on blue ice packs.
Third party independent comparisons of HyMax to other products:
Figure-1 Analysis of cloning factor supplements for production of IgG-producing B cell hybridomas. Splenocytes were fused with SP2/0 cells, cultured for 14 days before analysis of supernatant from the cultures. Analysis was performed via anti-Fc (gamma) ELISA. IgG positive clones were identified as those producing more than 25ng/mL IgG.
If your culture is having poor viability or productivity, we also offer HybriCare™ 20x, our proprietary Hybridoma Rescue Media (Item 113003). HybriCare™ includes additional growth factors and several antibiotics with HyMax™ as the basal media. It is shown to rescue many hybridomas both in terms of viability and improving productivity. HybriCare™ contains anti-mycoplasma antibiotics, one common reason for loss of many cell lines.
If mycoplasma contamination is confirmed in your culture, we have MycoLytixä 1000x, a ready to use mycoplasma inhibitor solution (Item 113015) to rescue such cultures.
We also offer guaranteed cell line rescue as custom service. We do all the work for you. Check our website to see our complete list of cell culture and hybridoma reagents. Contact us for more information.
MSDS is available here.