Product Name: HybriCare™ Hybridoma Rescue Media Supplement, 20x
Catalogue No.: 113003
Description: HybriCare™ Hybridoma Rescue Media Supplement, is a proprietary blend of growth factors, cytokines, hormones & antibiotics, when added to your current media at 5% will help rescue your clone from poor viability or productivity.
HybriCare™ is based on our HyMax™, our proprietary Hybridoma Supplement, as basal media. Hybridoma clones tend to lose viability or productivity spontaneously and randomly for various reasons. These includes loss of gene expression and non-obvious contamination such as mycoplasma. Using our extensive cell culture experience, we have specially formulated this media that contains many cytokines, growth factors and hormones which helps B-cells to retain their IgG isotype phenotype. It also contains some antibiotics to stop the invisible contamination such as mycoplasmales, mycotics & some bacteria. We are successful at rescuing over 80% of cell lines provided to us. It is prepared fresh and intended to be used within 6 months.
We also offer guaranteed hybridoma culture rescue as a custom service; we do all the work for you and you pay only if we are able to rescue your clone. Contact us for more information.
Usage: Add 5mL of HybriCare™ to 95mL of your media currently being used (including any supplements or antibiotics) for culturing cells. Do not use HybriCare™ at more than 10%. Continue with culturing cells with this media until cells have grown well for few generations. If microbial contamination is suspected use HybriCare for at least 6 generations before weaning off. Once cells have grown well, culture may be weaned off of HybriCare by gradually reducing the percentage in steps to 1%, 0.5% and 0%.
Format: Provided as a 20x concentrate ready to use solution. Never frozen before shipping to customer. Each lot is tissue culture tested.
Storage: It is recommended to store at 4°C - 8°C in refrigerator until use within 6 months. It can be made into aliquots and frozen stored at -20°C - 80°C for long term to use within 12 months period. Avoid repeated freezing and thawing, which may cause activity loss and or show precipitation.
Stability: 6 months when stored at 4°C - 8°C in refrigerator or 12 months when stored frozen. Once thawed use within 30 days or aliquot and store as needed. Do not freeze & thaw repeatedly.
Shipping: It is prepared fresh (within 30 days) and shipped in liquid form on ice packs.